Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Direct Human Basophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Basophil Isolation Kit isolates functional, highly purified basophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
Sigma Aldrich Fine Chemicals Biosciences Lipase from porcine pancre25G
LIPASE Lipase from porcine pancre25G
Sigma Aldrich Fine Chemicals Biosciences tobaco etch virus TEV Proteas
The tobacco etch virus (TEV) protease is a useful tool for the removal of fusion tags from recombinant proteins.
STEMCELL Technologies RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, For processing: 1000 mL cord blood processed
The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium. Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).
STEMCELL Technologies EasySep™ Human Th17 Cell Enrichment Kit II, For processing: 2 x 10^9 cells
The EasySep™ Human Th17 Enrichment Kit II is a two-step method designed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. First, CCR6+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the cells and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction is enriched for IL-17-producing Th17 cells.This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This kit replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554).For highly purified particle-free cells, we recommend the EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656). (https://www.stemcell.com/products/easysep-release-mouse-pe-positive-selection-kit.html)
STEMCELL Technologies EasySep™ Human Basophil Isolation Kit, For isolating: 1 x 10^9 cells
The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
Sigma Aldrich Fine Chemicals Biosciences High Pure Plasmid Isolatio
Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.Capacity The High Pure Spin filter Tubes hold up to 700 muL sample volume.Sample 0.5 - 4.0 mL cultures of E. coli (harvested at a density of 1.5 - 5.0 A 600 units/mL)
Biotium HCG-intact(HCGab/52), CF647 conjugate, 0.1mg/mL
This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.Primary antibodies are available purified, or with a selection of fluorescent CF
STEMCELL Technologies RoboSep™ Rat CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Rat CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Biotium Cytokeratin 18(DA7), CF594 conjugate, 0.1mg/mL
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower flu
Genscript Corporation GST Fusion Protein Purification Kit
Glutathione Resin (Cat. No. L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications.
STEMCELL Technologies EasySep™ Human "Do-It-Yourself" Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human "Do-It-Yourself" Positive Selection Kit II is designed to select any human cell type of interest with your own mouse IgG1 monoclonal antibody. Cells that express the surface antigen that is recognized by the mouse IgG1 monoclonal antibody are targeted for magnetic separation using EasySep™ Dextran RapidSpheres™ and the EasySep™ Magnet. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding.This product replaces the EasySep™ Human "Do-It-Yourself" Selection Kit (Catalog #18099).
Cayman Chemical MItoCheck MItochondrIalTIs 1ea
When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman’s MitoCheck Mitochondria (Tissue) Isolation Kit includes a complete package of reagents and step-by-step instructions for isolating coupled mitochondria* from freshly harvested mammalian heart, liver, or kidney tissue through a process of differential centrifugation in isotonic buffers. *Coupled mitochondria are defined as mitochondria capable of phosphorylating ADP at the F1FO-ATP synthase (Complex V) through the utilization of a proton (H+) gradient, which is generated by the electron transport chain (ETC) in the presence of appropriate substrates (e.g., succinate) and in the absence of uncouplers and mitochondrial inhibitors.