Protein Purification Reagents and Kits
New England Biolabs, Inc. Protein A Magnetic Beads 1 ml
Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. Specifically, the beads consist of a truncated form of recombinant Protein A that is covalently coupled to a nonporous superparamagnetic particle. The protein is coupled through a linkage that is stable and leak resistant over a wide pH range.
Invent Biotechnologies Inc Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes - Spin Colum-Based (20 Preps)
Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes are essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into nucleus and cytosolic fraction is a common practice in the lab. However, when it comes to adipocytes, separation of these two fractions is much more difficult because of the high concentration of lipid droplet and low protein content of adipocytes. Methods reported in the literature are tedious and time consuming and as much as 50 grams of tissue are required. This kit provides a very rapid and simple method for obtaining high purity nuclei from adipose tissues and most important of all, only milligram amounts of tissues are required making it possible to isolate nuclei and cytosol from small animal and biopsy samples.
Invent Biotechnologies Inc Minute™ ER Enrichment Kit - spin column-based (20 Preps)
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. ER plays a central role in exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in cytoplasm are translocated into ER from where vesicles shuttle protein cargo to the Golgi apparatus, which subsequently fuses with plasma membrane. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming, and contain significant cross-contamination. . Unlike any other ER isolation kit on the market, our ER isolation kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of starting cultured cells or tissues. This kit can differentially precipitate native ER from cultured cells/tissues without using a Dounce homogenizer or ultracentrifugation.
STEMCELL Technologies EasySep™ Human Th17 Cell Enrichment Kit II, For processing: 2 x 10^9 cells
The EasySep™ Human Th17 Enrichment Kit II is a two-step method designed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. First, CCR6+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the cells and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction is enriched for IL-17-producing Th17 cells.This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
Invent Biotechnologies Inc Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes, 20 preps, spin-column-based, detergent-free extraction, high yield 2-3 mg/ml, 50-80 mg adipose tissue
Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes, 20 preps, spin-column-based, detergent-free extraction, high yield 2-3 mg/ml, 50-80 mg adipose tissue
KAYAKU ADVANCED MATERIALS INC MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
Active Motif Siliconized Tubes, 1.7 ml
Because polystyrene transfers sonic wave energy much more efficiently than polypropylene, Active Motif highly recommends that its Polystyrene Sonication Tubes be used with its Q800R Sonicator. Use of polypropylene tubes in indirect sonication has been shown to reduce the extent to which the chromatin is sheared
Macherey-Nagel Protino Ni-NTA Agarose, 100 mL
Protino Ni-NTA Agarose (100 mL) Protino Ni-NTA suspension for the purification of His-tag proteins pack of 100 mL settled beaded agarose
Cayman Chemical GlutathIon ethyl ester 100mg
A cell-permeable derivative of GSH; increases mitochondrial GSH levels in C38 and IB3-1 epithelial lung cystic fibrosis cells at 10 mM; decreases reactive oxygen species (ROS) levels and TNF-α-induced IL-8 secretion in IB3-1 cells at 10 mM; increases mitochondrial complex I function and reduces depolarization of the mitochondrial membrane potential in IB3-1 cells at 10 mM and 1mM, respectively; decreases lipid oxidation and ferroptosis in cystine-deficient PANC-1, AsPC-1, BxPC-3, and S2-013 pancreatic cancer cells; increases the viability of irradiated human lymphoid cells at 5 mM
STEMCELL Technologies RoboSep™ Mouse NK Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse NK Cell Isolation Kit targets non-NK cells by labeling unwanted cells with biotinylated antibodies and EasySep™ Streptavidin RapidSpheres™, and separates cells without columns using an EasySep™ magnet. Desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture or cell-based assays.This product replaces the EasySep™ Mouse NK Cell Enrichment Kit (Catalog #19755) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse CD90.1 Positive Selection Kit, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD90.1 Positive Selection Kit is designed to isolate CD90.1+ (Thy1.1+) cells from single suspensions of splenocytes, lymph nodes and peripheral blood by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.1 and dextran-coated magnetic particles. Labelled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
STEMCELL Technologies EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion, For processing: 1 x 10^9 cells
The EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion is designed to isolate hematopoietic progenitor cells from fresh or previously frozen cord blood and other cell preparations that contain large numbers of platelets by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Vector Laboratories Glycoprotein Eluting Solution for Mannose/Glucose Binding Lectins | 100mL
Vector Laboratories has developed Glycoprotein Elution Solutions in the neutral pH range that maximize the yield of eluted glycoproteins and preserve the activity of the agarose-bound lectins for repeated use. All components of these ready-to-use Glycoprotein Eluting Solutions can be subsequently removed by dialysis. These Glycoprotein Eluting Solutions offer researchers convenience and superior recovery over standard sugar solutions.